Fig. 4. rBM increases ER mRNA levels in mammary epithelial cells. ER mRNA was analyzed by quantitative RT-PCR 2 and 6 days after isolating primary cells from the mammary gland (A), or in Scp2 cells cultured for 3 days (B) on plastic or in the presence of rBM (dripped or on top). Values were normalized with respect to GAPDH mRNA. Three independent experiments comprising different primary culture preparations or Scp2 cultures were used to create the graph. *P<0.05 vs. plastic for each condition.

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