Fig. 1. Survivin can be depleted from HeLa cells using RNAi. (A) Growth curve of viable HeLa cells transfected with control siRNA (blue triangles) or Survivin siRNA (red circles). Cell number in Survivin RNAi population does not increase after 48 hours. (B) Immunoblot analysis of control (C) and Survivin-siRNA-transfected (S) cultures at 0 hours, 60 hours or 84 hours post transfection as indicated. Tubulin was monitored as a loading control. (C-E) Indirect immunofluorescence images showing Survivin in red, DNA in blue and tubulin in green. (C) Control cell transfected with rhodamine-labelled control siRNA (shown in green). Survivin (red) localises normally to the centromeres. (D) Survivin-positive cell from same culture as E, showing abundant Survivin at the centromeres. (E) Survivin-depleted cell from a Survivin siRNA-transfected population, fixed and stained at 60 hours. No Survivin (red) is detectable at the centromeres. Bars, 5 µm.

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