Fig. 3. Survivin depletion causes loss of Aurora-B and INCENP from the centromeres. (A-D) Survivin-siRNA-transfected cells were fixed at 60 hours and stained for Survivin, Aurora-B, INCENP and centromeres (using ACA). (A,B) Survivin versus Aurora-B. (A) Survivin-positive cell shows Survivin (A') and Aurora-B (A") at the centromeres. (B) The Survivin-depleted cell (B') shows Aurora-B diffusely localised with some residual staining on the chromosome arms (B"). Merged images do not show Survivin staining. (C,D) INCENP versus Aurora-B. (C) Survivin-positive cell, as judged by the presence of Aurora-B staining at the centromeres (green, C') shows centromeric INCENP staining (C"). (D) The Survivin-depleted cell shows no Aurora-B (green, D') or INCENP (D") at the centromeres. (E) A plot of the intensity of Survivin versus Aurora-B at centromeres revealed a linear correlation between the abundance of the two proteins. (F) A plot of the intensity of Aurora-B (to indicate Survivin depletion) versus INCENP at centromeres revealed a linear correlation between the abundance of the two proteins. (E,F) Units indicate fluorescence intensity (x10³). Bars, 5 µm.

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