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Fig. 2. Fluorescence in situ hybridization of centromere probes to chromosomes in
syn1 meiocytes. Hybridization of a centromere probe (pAL1) was
detected with fluorescein-labeled streptavidin (green). Chromosomes were
stained with PI (red). (A) An example of an early leptotene meiocyte showing
six centromere signals; (B) zygotene; (C) diplotene; (D) diakinesis; (E)
metaphase; (F) telophase I. Assignment of the stage of the cell cycle is
approximate and based both on chromosome morphology and the stage of
surrounding cells in the anther. Bar, 5 µm.