Fig. 5. Calcium responses in T. gondii during gliding and cell invasion. (A) Calcium fluxes in gliding parasites. Fluo-4 AM loaded parasites were observed by time-lapse fluorescent video microscopy, with both phase and fluorescent images acquired with 0.5 seconds between each pair. Shown are selected images that were merged, with the elapsed time between frames indicated. Scale bar, 5 µm. (B,C) Merged images of fluo-4-labeled parasites during invasion into fibroblasts. Shown are selected merged pairs of images, with the elapsed time between subsequent frames indicated. Scale bar, 5 µm. Also see Movie 4 at jcs.biologists.org/supplemental.

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