Fig. 4. H. pylori induction of MMP-7 in AGS cells is associated with stimulation of migration and invasion. (A) Western blots indicate that addition of H. pylori to cultured AGS cells with a multiplicity of infection [MOI] of 100 (centre lane) or 200 (right lane) produced a graded increase in the abundance of MMP-7; the latter co-migrated with the 19 kDa human recombinant MMP-7 (arrow). (B) H. pylori (hatched bars), increased migration of AGS cells through Transwell filters. The number of migrating cells per field are presented; mean±s.e.m., n=3. (C) H. pylori (hatched bars) increased invasion of AGS cells through Matrigel-coated Transwell filters. The sum of the invading cells in five fields is presented; mean±s.e.m., n=3. Neutralization of MMP-7 using an MMP-7 monoclonal antibody (4 µg ml^-1) inhibited both migration and invasion.

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