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Fig. 2. ${gamma}$ -HIS2AV staining in wild-type female meiosis appears after the initiation of SC formation. All images are single sections, with ${gamma}$ -HIS2AV (red), C(3)G (green) and DNA (blue) staining. (A) An early region 2a pro-oocyte in zygotene with no nuclear ${gamma}$ -HIS2AV staining. (B) A pachytene nucleus with five ${gamma}$ -HIS2AV foci associated with the C(3)G-staining. A color merge and the ${gamma}$ -HIS2AV channel are shown. (C) A pro-nurse cell with ${gamma}$ -HIS2AV foci and fragments of C(3)G staining at the same developmental stage as the cell in B. The three ${gamma}$ -HIS2AV foci are all associated with segments of C(3)G staining. Next to this cell and in the same cyst is a pro-oocyte in pachytene. Bars, 1 µm.

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