Fig. 3. Induction of MHC class I by intracellular expression of IFN is abolished by removal of the NLS. WISH cells were transduced for 1 hour with an empty vector control (dotted line), a vector expressing non-secreted IFN (dark grey) or a vector expressing non-secreted IFN mutated in NLS (light grey). Cells were then allowed to grow for 48 hours in regular medium followed by staining with R-PE-conjugated monoclonal antibody to human MHC class I and analysis by flow cytometry. R-PE-conjugated murine IgG2a was used as a control. A similar profile was noted in three independent experiments.

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