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Fig. 5. Association of activated STAT1, IFN{gamma} and IFNGR1 with nuclear importer, NPI-1. Cell extracts from WISH cells transduced for 8 hours with an empty vector (lane 1), NLS-mutated IFN{gamma} expression vector (lane 2) or non-secreted IFN{gamma} expression vector (lane 3) were used for immunoprecipitation with an antibody to NPI-1. Equal amounts of immunoprecipitates were electrophoresed and probed individually with antibodies to IFNGR1 (first row), IFN{gamma} (second row), phospho-STAT1 (third row) or NPI-1 (fourth row).





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