Fig. 7. The effect of DYRK1A overexpression on the distribution of SC35 splicing factor is dependent on DYRK1A kinase activity. (A) A fluorescent image of a cell overexpressing GFP-DYRK1A, where nuclear speckles for both DYRK1A (green) and endogenous SC35 (red) appeared diffuse, and an untransfected cell showing the normal distribution of SC35. The nuclei were counterstained with DAPI (right). The GFP-fusion proteins of DYRK1A (B), as well as DYRK1B and a chimeric protein, DYRK1B/DYRK1A, with the C-terminal regions exchanged (C) were transfected and cells immunostained with anti-SC35. The percentage of transfected cells, which presented accumulation in speckles for the endogenous SC35 splicing factor, is represented in the charts to establish the extent of speckle disassembly induced by DYRK1A overexpression. The data are the average±s.d. of two independent experiments with at least 250 cells counted in each. Images of cells overexpressing DYRK1B, and the chimeric DYRK1B/A, co-stained with SC35, are also shown (C, right panel).

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