LFA-1-induced T cell migration on ICAM-1 involves regulation of MLCK-mediated attachment and ROCK-dependent detachment
J Cell Sci
Smith et al. 116 (15): 3123.
JCS00606 Supplementary Data
Files in this Data Supplement:
Movie 1
-
Initial
interactions between T cells and ICAM-1 in the presence of Mg2+/EGTA
(magnification ´40) (1 second
video=1 minute real time).
Movie 2
-
Effect of
MLCK inhibitor ML-7 (20 mM) on T
cells migrating on ICAM-1 in the presence of Mg2+/EGTA
(magnification ´40) (1 second video=1
minute real time). The black frame indicates time of inhibitor addition.
Movie 3
-
Effect of
CaM inhibitor W-7 (50 mM) on T
cells migrating on ICAM-1. Other conditions as for Movie 2.
Movie 4
-
Effect of
ROCK inhibition by Y-27632 (20 mM)
on T cells migrating on ICAM-1 (magnification ´63
). (1 second video=2 minute real time). Other conditions as for Movie 2
Movie 5
-
Effects
of ROCK inhibition followed by MLCK inhibition on T cells migrating on ICAM-1
in the presence of Mg2+/EGTA. Y-27632 (20 mM) was included from the start and black
screen indicates ML-7 (25 mM) addition. (magnification ´40) (1 second video=1 minute real time).
Figure 1
-
(A)
The combined effect of Y-27632 and ML-7 on T cell attachment to ICAM-1 in the
presence Mg2+/ EGTA. Data are representative of n=3 with individual points the means of triplicate
samples. (B) The effect of RhoA inhibitor C3 exoenzyme on T cell attachment to
ICAM-1 in the presence Mg2+/EGTA. Data are representative of n=2, with individual points representing the means of
quadruplicate samples.
