Fig. 10. Effect of MLCK and ROCK inhibitors on migration of the T cell population. (A) Average speed of Mg²⁺/EGTA-stimulated T cells on ICAM-1 in the presence of inhibitors 25 µM ML-7, 50 µM W-7, 20 µM Y-27632 (all n=15) or no inhibitor (n=55). (B) The T cells were plated on ICAM-1 in the presence of Mg²⁺/EGTA and allowed to migrate for 20 minutes. Inhibitor treatment was initiated and the T cells were scored after a further 10 minutes. `Lamellar protrusion' describes membrane ruffling at either the leading edge or, in the case of stationary cells, around the cell circumference. `Tail anchored' describes polarized cells that failed to translocate a full cell length. n=50-70 per treatment group.

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