Fig. 3. Whole-mount immunolocalisation indicates that StPT2 is present in the elongation zone where it is confined to the epidermal layer, and in the root hairs. (A-E) Superficial staining pattern in root tips, and F shows optical section collected at 100 µm depth. (Arrow indicates root cap cells.) (G) Projected image of quartz-grown plants to show labelling in the root hairs. Roots were excised from P-depleted (A, C-G) or P-supplied (B) plants and labelled with -tubulin (A-F) antibody (green) and StPT2 (A,B,F,G), pre-immune (C) VPPase (D) and ATPase (E) antibodies (red). Yellow indicates colocalisation of red and green. Bar, 100 µm.

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