Fig. 4. StPT2 is outside -tubulin labelling but inside cell wall labelling. P-depleted root whole-mount tissue from the tip region viewed at a depth of 10 µm, labelled with -tubulin (A-C), cell wall (D) (green) and StPT2 (A and D), plasma membrane ATPase (B) and VPPase (C) (red). In A and B, dotted arrow (CS, cross-section) indicates cell with peripheral red labelling where the optical section cuts across a region of the cell with label around its circumference. Single arrow (EF enface), cell with red labelling covering the green labelling where the optical section passes above the cell. Double arrow indicates cell without StPT2 labelling. In D, solid arrow is plasma membrane (PM) and dotted arrow, cell wall, (CW). Bars, A and B, 25 µm; C and D, 16 µm.

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