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Fig. 5. Image of root transverse sections showing that StPT2 labelling is confined
to the apical plasma membrane of epidermal cells, whereas the plasma membrane
ATPase labels the whole cell periphery of epidermal and outer cortical cells.
-Tubulin labelling is across the whole root. Transverse sections (50
µm) were taken of root whole mount 700 µm from the tip (apart from C,
300 µm) labelled with StPT2 (A,C,D,F) and plasma membrane ATPase (E,G)
(red) and
-tubulin (green) (B,C,F,G). Double dotted arrow indicates
labelling with StPT2 of the apical plasma membrane of the epidermal cells.
Single arrowhead shows some ATPase labelling in the cytoplasm; double arrow
shows possible enface view of a cell. Bars, A and B, 50 µm; C, 100 µm;
D-G, 16 µm.