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Fig. 6. Prenylated PSD-95 does not multimerize with itself or with palmitoylated PSD-95. COS cells were transfected with plasmids encoding GFP- and FLAG-tagged PSD-95 constructs, some of which contain the paralemmin (PSD-95-prenyl) C-terminal consensus sequence for isoprenylation. Cells were lysed with 1% Triton X-100 and proteins were immunoprecipitated with an antibody to GFP. Western blotting reveals that PSD-95-prenyl multimerizes neither with itself nor with wild-type (palmitoylated) PSD-95.





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