Fig. 2. ZnCl₂ potentiates PMA-stimulated eosinophil depolarization. Transmembrane potential was determined using diBAC₄(3) and flow cytometry. Absolute membrane potential was calculated as described in Materials and Methods. Eosinophils were stimulated at room temperature with 800 nM PMA in the absence (; n=4) or presence (; n=4) of 250 µM ZnCl₂. ZnCl₂ was added immediately before stimulation. Mean values±s.e.m. are shown; where the error bars are not visible they are smaller than the symbol.

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