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Fig. 2. The strong signal for NDP kinase around vesicles and in ruffling membranes is not a volume effect. Fluorescence ratio imaging of cells transfected with GFP and stained with anti-NDP kinase-A after treatment with 10 ng ml–1 EGF for 1 minute. (a) NDP kinase A, (b) GFP and (c) corresponding phase-contrast image. (d) Ratio image showing high NDP kinase A/GFP ratios (arbitrarily set as dark areas) at edge ruffles and around large vesicles in the perinuclear area. Scale bar, 25 µm.





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