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Fig. 8. Characterization of the vesicles labeled by NDP kinase. (A). Size
distribution of NDP kinase-labeled vesicles. The histogram was built as
described in Materials and Methods. (B) NDP kinase is associated with vesicles
labeled by LAMP-1. This image shows the immunolocalization of NDP kinase with
Ab-1 (red) and LAMP-1 (green) in quiescent cells. NDP kinase labels the
periphery (a) of vesicles, whereas LAMP-1 is visible in the lumen (b, arrows);
a circular ruffle (arrowhead) is stained only by NDP kinase. Scale bar, 25
µm.A large vacuole positive for both NDP kinase (c) and LAMP-1 (d) has a
multivesicular appearance. Scale bar, 5 µm.
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