Fig. 7. Pancreatic acinar cells expressing a dominant negative Mist1 protein lack Cx32-containing gap junctions. Transgenic mice expressing the Mist1^{mut basic} protein in acinar cells were analyzed for Mist1^{mut basic} expression as well as for expression of Cx32. (B,D) The Mist1^{mut basic} protein contains a myc epitope tag and can be detected using a myc antibody. (C,D) Acinar cells expressing the Mist1^{mut basic} protein (red, arrowhead) do not accumulate Cx32-containing gap junctions (green, arrows), whereas acinar cells lacking the Mist1^{mut basic} protein retain Cx32 expression. Nuclei in A are stained with the DNA fluorochrome DAPI (blue).

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