Fig. 1. Intracellular localization of human HP1 subtypes. (A) Localization of human HP1 subtypes in interphase nuclei. HeLa cells transiently expressing YFP-HP1 (a-e), YFP-HP1ß (f-j), or YFP-HP1 (k-o) were fixed, and centromeres and PML nuclear bodies were viewed by indirect immunofluorescent staining. (b,g,l) centromeres, (c,h,m) YFP-HP1 and (d,i,n) PML nuclear bodies in the same cells. Merged images in a, f and k, of HP1 (green) and of centromeres (red) and in e, j and o for HP1 (green) and the PML nuclear bodies (red). Bar, 10 µm. (B) Metaphase localization of HP1 subtypes. HeLa cells transiently expressing YFP-HP1 (a-c), YFP-HP1ß (d-f), or YFP-HP1 (g-i) were fixed and stained with DAPI (a, d and g). (c, f, and i) Merged images of HP1 (green) and DAPI (red). Bar, 10 µm. (C) HeLa cells transiently expressing YFP-HP1 were arrested at mitosis by treatment with 650 nM nocodazole for 8 hours. Centromeres were stained with human antisera that recognizes CENP-A, CENP-B, and CENP-C proteins (Masumoto et al., 1989). (a) DAPI, (b) YFP-HP1 and (c) centromeres. (d) A merged image of DAPI (blue), YFP-HP1 (green) and centromeres (red). (e) An enlarged image of the boxed area in d showing YFP-HP1 (green) and centromeres (red). Scale bar, 10 µm.

Return to article