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Fig. 5. Domains of HP1
required for its localization. HeLa cells transiently
expressing HP1 fragments fused with YFP and the CENP-B N-terminal
region fused with CFP. (A) Diagram of YFP- HP1 truncation constructs
and their intracellular localization: +, localized; –, not localized;
+/–, ambiguous; and ND, not determined. Quantitative data were obtained
from three independent experiments. (B) YFP- HP1 fragment localization
during metaphase: YFP- HP1 fragment (left column), CFP-CENPB-N-terminal
fragment (middle column), and merged images (right column). (C) Localization
of HP1 fragments in the interphase nucleus. (D) Localization of
HP1 and PML nuclear bodies. Scale bar, 10 µm.
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