Fig. 2. Dynamic localization of the Nuf2-Hec1 complex during the cell cycle. (A) Localization of Nuf2-GFP at progressive stages of the cell cycle in DT40 cells. Cells were fixed and stained with anti-CENP-C antibody (red). Green signals are specific for Nuf2-GFP. Nuclei and chromosomes are counterstained with DAPI (blue). As shown in the merged images, Nuf2-GFP signals begin to colocalize with CENP-C signals in G2 and are colocalized with CENP-C throughout mitosis. (B) Cells that express Nuf2-GFP (green) were fixed and stained with anti--tubulin (red). Nuclei and chromosomes are counterstained with DAPI (blue). (C) Selected images of chromosomes (Hoechst; top row) and Nuf2-GFP (middle row) in living cells. In the superimposed images (lower row), chromosomes and Nuf2-GFP are displayed in red and green, respectively.

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