Fig. 6. Chromosome and microtubule dynamics in Nuf2-deficient cells. (A) Selected images of chromosomes (upper) and microtubules (middle) in Nuf23-63 cells (–tet) from prophase to telophase. In the merged images (lower), chromosomes and microtubules are displayed in red and green, respectively. The numbers at the top of each image represent the time in minutes. (B) Observation of a single cell beginning 18 hours after addition of tet. Selected frames are shown. Once the cell entered mitosis, it arrested at prometaphase for approximately 400 minutes. The cell died during the mitotic stage between 380 and 460 minutes.

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