Fig. 2. Endocytosed F-Dex and Cy3mBSA colocalize in Rab7-containing endosomes at late times but are present in Rab-7-negative structures at early times. (A-C) Hemocytes incubated with F-Dex (A, green in C) and Cy3mBSA (B, red in C) for 5 minutes and chased for 2 minutes, and imaged on a wide-field microscope, colocalize in large centrally located endosomes (C, arrows). (D-H) Confocal microscopy shows that at 5 minutes the majority of F-Dex-containing endosomes (D, arrows; green in F) colocalize with Rab7 (red in F), whereas in a 45 seconds pulse of F-Dex (H, green; arrowheads) and Alexa488 mBSA (G, green; small arrows) do not colocalize with anti-Rab7 (red in G and H, arrows). Insets show magnified view of the area marked by an asterisk. Bar, 5 µm; inset, 1 µm.

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