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Fig. 5. GFP-GPI is internalized into LR-Dex early endosomes and Cy5mBSA early
endosomes. Hemocytes from Collagen-Gal4;UAS-GFP-GPI (A-D) were incubated with
Fl-anti-GFP (pseudo-colored green; Cy5-anti-GFP in A,C and Alexa568-anti-GFP
in B,D) at room temperature for the indicated times in the presence of LR-Dex
(A,C; red) or Cy5mBSA (B,D, red). Note that Fl-anti-GFP is partially
colocalized with LR-Dex (A, arrowheads; C, arrows; insets) and with Cy5mBSA
(B, small arrows; D, arrows; insets) at these times. However, at late times,
all three probes colocalize in late endosomes (arrows, C,D). Insets (red, top;
green, middle; merge, bottom) show the area marked by the asterisk. Bar, 5
µm; inset, 1 µm.
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