Fig. 6. Endocytosis of Cy3mBSA is perturbed in shi^ts cells at a restrictive temperature while F-Dex uptake appears unaffected. (A) Schematic of Drosophila dynamin (PH, plextrin homology domain; GED, GTPase effector domain; PRD, proline-rich domain) showing the locations of the shi^ts2 (G141S) and shi^ts1 mutations (G267D). (B-J) Cells from wild-type CS animals (B,E,H) internalize Cy3mBSA (red) and F-Dex (green) into endosomes following a pulse for 5 minutes at 21°C (B, arrows) and 31°C (E, arrows). Cells from shi^ts1 (C,F,I) and shi^ts2 (D,G,J) animals internalize Cy3mBSA and F-Dex at 21°C (C,D, arrows). At 31°C in cells from shi^ts animals (F,G), Cy3mBSA remains peripherally distributed in punctate (small arrowheads) and hazy distribution, whereas F-Dex continues to be internalized into large endosomes (arrows). Cells labeled with Cy3mBSA for 20 minutes on ice (H,I,J) show a distribution similar to shi^ts cells at 31°C (small arrowheads). Bar, 5 µm.

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