Fig. 3. Stimulation of superoxide production by conditioned medium. Ax2 cells were starved in shaking suspension, and after 6 hours were resuspended at 10⁸ cells/ml in fresh buffer, in conditioned medium (CM), or heat-treated conditioned medium (CM h/t), all containing 0.5 mM XTT. After 10 minutes the absorbance at 470 nm was measured. XTT reduction in the presence of the relevant medium (buffer or CM) and the absence of cells was subtracted. The data are the means ± s.e.m. of three experiments.

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