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Fig. 3. Split centrioles organize functional spindle poles. (a) A RanBP1-RFP-transfected mitosis from centrin 1-GFP stably expressing L929 cell cultures. Centrin-1/GFP allows the visualization of centrioles (b); the spindle is stained with anti-{alpha}-tubulin, revealed with an AMCA-conjugated secondary antibody (c). Merging of b and c produces d, which depicts single split centrioles (green) at each spindle pole (AMCA-stained MTs, in blue). Scale bar, 10 µm.





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