Fig. 3. The response to IAA is dependent on SWE1. (A) A haploid 1278h MAT strain (wild type; wt) and a swe1 derivative were incubated for 16 hours in YEPD alone or YEPD plus 0.5% IAA, and photographed using DIC optics. No filaments were produced by the SWE1 mutant upon IAA treatment. Scale bar: 10 µm. (B) Flow cytometry traces showing relative DNA content in untreated 1278h cells, and cells exposed to 0.5% IAA for the indicated times. The proportion of cells in G1 and G2 was determined for each time point and the proportion of cells in G2 in the treated (open symbols) and untreated (closed symbols) cultures is plotted against time.

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