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Fig. 7. cAMP induces ß-casein expression in an adhesion-independent manner.
(A) Morphology of CID-9 cells on day 6 of culture, plated on PolyHEMA (Ctrl;
control) and supplemented with 50 µM 8-Br-cAMP (cAMP). (B) Western blot for
ß-casein, Cx43, Cx32 and Cx26 and for ß-actin for cells plated on
PolyHEMA (Ctrl; control) and supplemented with 50 µM 8-Br-cAMP (cAMP).
Significant increase in ß-casein expression (P<0.001) and
Cx43 (P<0.001) was noted when cells on polyHEMA were treated with
8-Br-cAMP compared with untreated cells.
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