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Fig. 5. Expression and ligation of 
6ß4 integrin changes the
localization and activation state of 3ß1 integrin. Cells were
cultured on glass coverslips for 4 days prior to immunofluorescence staining
for FA components. Cells were fixed with 3% formaldehyde and solubilized with
0.5% Triton X-100 buffer for 30 minutes at RT. Fixed cells were washed with
PBS and blocked with 1% BSA for 1 hour. Cells were stained with
FITC-phalloidin for filamentous actin (stained in green A-C) and anti-paxillin
mAb (stained in red A-C). Further samples were stained for 3 integrin
(D-F) and the conformationally active form of the ß1 integrin subunit
with mAb HUTS-4 (G-I). Scale bar; 20 µm.
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