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Fig. 6A,B,C. HSF2 affects the localization of HSF1 in stress granules. (A-C) HeLa cells
were transiently transfected with Flag-tagged HSF2 and constructs shown in
Fig. 4A. Cells were subjected
to a 1-hour heat shock (HS) or left untreated (C), and immunodetected with
anti-Flag antibodies for HSF2 deletion mutants (green) and with polyclonal
HSF1 antibodies for the endogenous HSF1 (red). Colocalization is shown in the
merge lane (yellow) and DNA is stained with DAPI (blue).
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