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Fig. 1. Role of VE-cadherin in cell spreading and proliferation. (A,B) Phase
contrast images of bovine pulmonary artery endothelial cells treated for 96
hours with anti-VE-cadherin (A) and control (B) antibodies. Scale bar: 50
µm. (C) Graph of mean projected area of endothelial cells with time after
treatment with anti-VE-cadherin and control antibodies. (D) Graph of number of
cells after treatment with anti-VE-cadherin and control antibodies, normalized
to number of cells at 24 hours after plating. (E) Flow cytometry diagrams of
cell-cycle distribution of unsynchronized cells (left) and synchronized cells
(right). Quantification of cell-cycle distribution is shown for each
condition. (F) Graph of percentage of cells in S phase (BrdU incorporation)
and mitotic index with time after replating of synchronized cells. (G) Graph
of mean projected area of endothelial cells as a function of seeding density
after treatment with anti-VE-cadherin and control antibodies. (H) Graph of
percentage of endothelial cells entering S phase (incorporating BrdU) as a
function of seeding density after treatment with anti-VE-cadherin and control
antibodies. Error bars represent the s.d., with (*)
P<0.05 relative to controls as calculated by t-test.
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