Fig. 4. Role of cell spreading in contact-mediated inhibition of proliferation. (A) Graph of percentage of fully spread cells for single cells and pairs of cells cultured in wells of 1500 µm²/half, 2000 µm²/half and 2500 µm²/half (left); percentage of fully spread endothelial cells when co-cultured with null or VE⁺ cells in wells of 1500 µm²/half (right). (B) Graph of percentage of cells entering S phase for single cells and pairs of cells cultured in all three sizes of wells. (C,D) Graph of percentage of cells entering S phase for single cells and pairs of cells cultured in all three sizes of wells when cells were separated into (C) fully or (D) partially spread populations. Error bars indicate the s.d. of four experiments, with (*) P<0.05 relative to single cells, as calculated by the paired Student's t-test.

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