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Fig. 2. Enlargement of synaptic vesicles caused by dysfunction of Rab5. (A-C) Nerve terminals of the photoreceptor cells from wild-type (A) and Rh1-GAL4/UAS-Rab5N142I mutant (B,C) flies. (D) A nerve terminal of the photoreceptor cell expressing antisense Rab5 RNA (GMR-GAL4/UAS-antisenseRab5). Inset, immunoblot analysis of the expression of Rab5 in the eyes of the wild-type (WT) and the GMR-GAL4/UAS-antisenseRab5 (AS) flies. (E) A typical nerve terminal of the Rh1-GAL4/UASRab5Q88L mutant. (F) A nerve terminal of Rh1-GAL4/UAS-EGFP-Rab5. The arrowheads and arrows indicate enlarged and tubular synaptic vesicles, respectively. Scale bar, 250 nm.





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