Fig. 7. dRheb is required for cell and tissue growth. As controls, wild-type eyes and heads (A,B) and sections of wild-type eyes viewed by light microscopy (C) are shown. dRheb loss-of-function clones (dRheb^-/-) were detected only when generated in a Minute (M^+/-) background. In this M^+/- background, eyes and heads composed primarily of dRheb^+/+,M^+/+ clones (white tissue), generated by FLP-FRT mediated recombination in a M^+/- background, are approximately the same size as those of wild-type (A,B,D,E). Eyes and heads containing dRheb^-/-,M^+/+ clones are dramatically reduced in size (D-I; white tissue is dRheb^-/-, red tissue is dRheb^+/-). Sections of these eyes show disorganized, smaller ommatidia composed of smaller cells (C,J; arrow indicates small, Rheb^-/- photoreceptor cell, arrowhead indicates normal size, Rheb^+/- photoreceptor cell). A,D,H, lateral view of eye; B,E,I, frontal view of head; F,G, dorsal view of head.

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