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Fig. 1. Rapid accumulation of PtdIns(3,4,5)P3/PtdIns (3,4)P2 at the T. cruzi invasion site. Fluorescence images of (A) CHO cells or (B) primary rat cardiomyocytes transiently expressing Akt-PH-GFP following incubation with infective T. cruzi trypomastigotes for 15 minutes. (C) T. cruzi invasion of CHO cells expressing Akt-PHGFPR25C which fails to bind to PtdIns(3,4,5)P3/PtdIns(3,4)P2. Extracellular parasites were stained with T. cruzi-specific antibodies in non-permeabilized cells. Host cell and parasite DNA were visualized with DAPI (A-C, blue). (D) Recruitment of Akt-PH-GFP to membranes surrounding invading T. cruzi trypomastigotes is sensitive to wortmannin. The relative number of Akt-PH-GFP-associated parasites was determined in CHO cells infected for 15 minutes following treatment with 20 nM wortmannin or vehicle control for 30 minutes. Data are represented as means ± s.d. n=3.





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