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Fig. 6. Inhibition of host cell PI 3-kinases during T. cruzi invasion blocks the lysosome-mediated entry pathway. (A,B) CHO cells expressing PLC{delta}-PH-GFP were treated with 40 nM wortmannin or vehicle control for 30 minutes prior to incubation with trypomastigotes for 15 minutes. Fixed permeabilized cells were stained with antibodies to lamp-1. The relative number of (A) intracellular parasites and (B) lamp-1 or PLC{delta}-PH-GFP-positive intracellular parasites was determined. (C) The relative number of intracellular parasites determined following T. cruzi infection of p85{alpha}+/-ß-/- ({alpha}HET) and p85{alpha}-/-ß-/- (KO) MEFs 15 minutes. (D) Kinetics of lamp-2 association with intracellular T. cruzi in {alpha}HET and KO cells at 10, 20, 30, 45, and 60 minutes post-infection. Data are represented as means ± s.d.; n=3.





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