Fig. 4. Binding partners of Pex10p. (A,B) Cells containing Pex10-C_ub-RUra3p and expressing the indicated N_ub fusion proteins were diluted to an OD₆₀₀ of 1, and 4 µl of this and of tenfold serial dilutions were spotted onto media containing 100 µM CuSO₄ and either lacking uracil (A) or containing both uracil and 5-FOA (B). Both media were lacking tryptophan and histidine to select for the plasmids. Cells were incubated at 30°C for 48 hours. (C) Yeast cells co-expressing Pex10p tagged with a six-fold repeated ha-epitope (Pex10-6ha) together with either Pex4p, Pex10p, Pex12p, Pex11p or Pex22p, all bearing a ninefold repeated myc-epitope at their C-termini, were subjected to a co-precipitation using the anti-ha-antibody to precipitate and the anti-MYC antibody to detect the co-precipitated protein after SDS-PAGE and transfer to nitrocellulose (C). (D) as in (C) but using anti-myc antibody for precipitation and anti-ha antibody for the detection of the co-precipitated Pex10-6ha. The asterisk indicates the heavy chain of the antibody used for the precipitation.

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