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Fig. 4. Zymographic analysis of medium conditioned by MPCs (300,000 cells per culture well) without (control) or with Ang-1 (0.5 µg ml-1), Ang-2 (0.5 µg ml-1) or the Ang-1/Ang-2 combination (A). Bar graph shows quantitative evaluation of gelatinase bands by densitometry (B). Optical density values were normalized for the total protein content of each sample. Notice that Ang-1 and Ang-2 used alone or in combination stimulated MMP-2 production by MPCs.





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