Fig. 3. Misregulation of lamin A/C and NuMA expression in one-cell-stage NT embryos. (A) Reconstructed embryos were activated for 6 hours with SrCl₂ and either no supplementation (-), 20 µg ml^-1 cycloheximide (CHX) or 5 µg ml^-1 actinomycin D (ActD). At the end of incubation, embryos were analysed by immunofluorescence. Anti-lamin-B (rabbit polyclonal) and anti-lamin-A/C (mAb) antibodies were used on the same preparations. Insets, DNA. Scale bar, 20 µm. (B) Relative immunofluorescence labeling intensities for indicated markers in nuclei of NT embryos treated as in (A). Reference value for each marker (100% fluorescence) represents relative amounts of immunolabeling in untreated embryos. n15 embryos per marker per treatment.

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