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Fig. 4. Changes in GSK-3 phosphorylation induced by staurosporine. The cells were allowed to spread on fibronectin for 120 minutes and then treated with 50 nM staurosporine for either 0-60 minutes or left untreated for 60 minutes (C60'). The cell lysates were separated by SDS-PAGE and immunoblotted with antibodies recognizing GSK-3{alpha}/ß phosphorylated on serine 21/9 (A,B), GSK-3{alpha}/ß phosphorylated on tyrosine 279/216 (C) or total GSK-3 (D). The bands visible in A represent GSK-3ß. GSK-3{alpha} phosphorylation followed a similar trend but was much weaker. P-GSK-3{alpha} is visible in B, which represents a longer exposure of A.





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