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Fig. 8. The role of PLC-
1 in E-lam formation. (A) To study the role of
PLC- in E-lam formation, the spread cells were treated with PLC
inhibitor U-71322 (0-4 µM) in the presence or in absence of staurosporine
and analyzed for cell spreading and E-lam formation. The results show the
mean±s.d. of one typical experiment (n=8).
(*P<0.05; **P<0.01;
***P<0.001.) (B) Immunolocalization of tyrosine
phosphorylated (active) PLC-1 (a,b) and actin (c,d) in control (a,c)
and staurosporine-treated (b,d) cells. PLC-1 and actin accumulated in
E-lams are marked with arrows and arrowheads, respectively. Scale bar: 10
µm.
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