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Fig. 2. Papilloma and squamous cell carcinoma formation. (A-B) Frequency of papillomas (A) and SCCs (B); ++++, all mice in group deceased. (C) Histopathological analysis of papillomas (pap) and SCCs. (D) Metastases from Inv{alpha}6ß4 mice. (C,D left-hand panel) Stained with haematoxylin and eosin; (D) other panels labelled with antibodies to the {alpha}6 transgene or keratin 14. Bar, 100 µm. (E) Immunolocalization of phosphoSmad2 in wt and Inv{alpha}6ß4{Delta}-transgenic epidermis, papillomas (pap) and SCCs; ace, acetone treated; TPA, 24 hours after one dose of TPA. Bars, 50 µm (epidermis), 100 µm (pap, SCC).





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