Fig. 7. Chlamydial migration is not inhibited by disruption of the dynactin complex due to overexpression of p50 dynamitin. Cos-7 cells were transiently transfected with GFP-dynamitin before infection with C. trachomatis or labeling with Alexa-Tf or Alexa-CTX. Cells expressing GFP-dynamitin (green in merged image) show no defects in chlamydial migration. The nascent inclusions stained with an antibody to L2 EBs (red in merged image) aggregate at a single perinuclear site in GFP-positive cells (arrowhead). Transiently transfected cells incubated with Alexa-Tf (red in merged image) show that Tf accumulated normally at a peri-Golgi region (arrowheads) in untransfected cells (no green signal in merged image) but Tf trafficking was inhibited in a neighboring cell expressing GFP-dynamitin (green cell in merged image). Similar results were observed for Alexa-CTX trafficking. CTX (red in merged image) was delivered to the Golgi (arrowhead) in the untransfected cell (no green signal in merged image) but delivery was abolished in GFP-dynamitin-expressing cells (green in merged image). Bars, 10 µM.

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