Fig. 9. The nascent inclusion recruits p150^(Glued) and this recruitment is not inhibited by nocodazole treatment or over expression of p50 dynamitin. Simultaneous visualization of p150^(Glued) and the chlamydial nascent inclusions by indirect immunofluorescent microscopy show colocalization of p150^(Glued) with the aggregated chlamydiae after infection for 5 hours. Inhibition of chlamydial migration with nocodazole does not block association with p150^(Glued). Furthermore, colocalization of p150^(Glued) and Chlamydia is not disrupted by overexpression of GFP-dynamitin (blue in the merged images). Bar, 10 µM.

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