Fig. 1. The C terminus of angiomotin contains a conserved putative PDZ-binding motif. (A) Eight amino acids of the C terminus of human and mouse angiomotin are aligned with glycophorin C, a protein with a characterized PDZ binding motif. The last four amino acids that determine the binding specificity to PDZ-containing proteins are boxed. (B) Coomassie staining of SDS-PAGE showing specific binding of a 90 kDa protein isolated by peptide affinity chromatography using the last 20 amino acids of angiomotin (lane 1). The protein did not bind the angiomotin peptide that lacked the C-terminal amino acids YLI (lane 2). (C) Generation of 3' deletion mutations of the angiomotin gene. A series of 3' deletions were generated by PCR amplifications from the angiomotin cDNA as described in the Materials and Methods. The deletions ranged from 20-3 amino acids and were cloned into the pBabe retroviral vector. (D) Western blot analysis showing expression levels of angiomotin and the 1-4 mutants after transfection into MAE cells.

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