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Fig. 3. Deletion mutants of angiomotin inhibit endothelial tube formation. Tube formation of vector, angiomotin and {Delta}1 and 4 transfected MAE cells was analyzed by in the matrigel assay. (A) Images show tube formation 16 hours after the start of the experiment. Scale bar: 300 µm. (B) Total tube length formed by vector, angiomotin and {Delta}1 and 4 transfected MAE cells. All analyses were performed in triplicate. The differences between vector and the {Delta}1 and {Delta}4 mutants were statistically significant (asterisks denotes P<0.001, error bars=s.d.).





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