Fig. 5. Progressive paralysis and loss of muscle filament organization with talin RNAi. (A) Time course of paralysis in talin-depleted animals (circles) or animals grown on bacteria carrying the empty RNAi vector (squares). Paralyzed animals were counted at 24, 48, 52, 58 and 72 hours. The error bars represent the standard deviation of at least 5 independent experiments. (B) At 72 hours, paralyzed animals are immobile except for occasional movement of the head. Staining with MH25 anti-PAT-3 monoclonal antibody was used to visualize dense bodies in body wall muscle cells of animals grown on bacteria with empty vector (C) and with talin RNAi (D) for 72 hours. Scale bars: 100 µm (B), 25 µm (C,D).

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